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sh‐cdk9 plasmid  (Genechem)

 
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    Structured Review

    Genechem sh‐cdk9 plasmid
    Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of <t>CDK9</t> and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, <t>E),</t> <t>sh‐CDK9</t> (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.
    Sh‐Cdk9 Plasmid, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sh+cdk9+plasmid/recombinant+lentivirus+sgk3+shrna/pmc05633544-29-10-15
    Average 90 stars, based on 1 article reviews
    sh‐cdk9 plasmid - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "miR‐206 inhibits the growth of hepatocellular carcinoma cells via targeting CDK9"

    Article Title: miR‐206 inhibits the growth of hepatocellular carcinoma cells via targeting CDK9

    Journal: Cancer Medicine

    doi: 10.1002/cam4.1188

    Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of CDK9 and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, E), sh‐CDK9 (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.
    Figure Legend Snippet: Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of CDK9 and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, E), sh‐CDK9 (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.

    Techniques Used: Over Expression, Expressing, Transfection, Stable Transfection, Control, Western Blot

    Inhibition of CDK9 by shCDK9 and LDC000067 represses the cell proliferation and induces apoptosis and cell cycle arrest in HCC cell lines. Bell7402 and HepG2 cells were transfected with sh‐CDK9 and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 for 48 h, then the cell proliferation (A and B) was examined by CCK‐8 assays. The apoptosis (C–J) and the cell cycle phase distribution (K–L) were detected by flow cytometry. * P < 0.05.
    Figure Legend Snippet: Inhibition of CDK9 by shCDK9 and LDC000067 represses the cell proliferation and induces apoptosis and cell cycle arrest in HCC cell lines. Bell7402 and HepG2 cells were transfected with sh‐CDK9 and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 for 48 h, then the cell proliferation (A and B) was examined by CCK‐8 assays. The apoptosis (C–J) and the cell cycle phase distribution (K–L) were detected by flow cytometry. * P < 0.05.

    Techniques Used: Inhibition, Transfection, Stable Transfection, CCK-8 Assay, Flow Cytometry



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    sh‐cdk9 plasmid  (Genechem)
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    Genechem sh‐cdk9 plasmid
    Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of <t>CDK9</t> and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, <t>E),</t> <t>sh‐CDK9</t> (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.
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    Image Search Results


    Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of CDK9 and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, E), sh‐CDK9 (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.

    Journal: Cancer Medicine

    Article Title: miR‐206 inhibits the growth of hepatocellular carcinoma cells via targeting CDK9

    doi: 10.1002/cam4.1188

    Figure Lengend Snippet: Overexpression of miR‐206, shCDK9, and LDC000067 suppresses the expression of CDK9 and its downstream genes. Bell7402 and HepG2 cells were transfected with GV251‐miRNA‐206 (A, E), sh‐CDK9 (C, D and F), and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 (G) for 48 h. Then the levels of CDK9 mRNA were assayed by qPCR (A, C) taking β‐actin as a control, and the protein levels of CDK9, p‐RNAII, t‐RNAII, and MCL‐1 were detected by western blot (B, D–G) taking tubulin as the loading control. * P < 0.05.

    Article Snippet: LDC000067 (CDK9 inhibitor) was purchased from Amquar Company (Shanghai, China). sh‐CDK9 plasmid was synthesized by GeneChem Company (Shanghai, China).

    Techniques: Over Expression, Expressing, Transfection, Stable Transfection, Control, Western Blot

    Inhibition of CDK9 by shCDK9 and LDC000067 represses the cell proliferation and induces apoptosis and cell cycle arrest in HCC cell lines. Bell7402 and HepG2 cells were transfected with sh‐CDK9 and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 for 48 h, then the cell proliferation (A and B) was examined by CCK‐8 assays. The apoptosis (C–J) and the cell cycle phase distribution (K–L) were detected by flow cytometry. * P < 0.05.

    Journal: Cancer Medicine

    Article Title: miR‐206 inhibits the growth of hepatocellular carcinoma cells via targeting CDK9

    doi: 10.1002/cam4.1188

    Figure Lengend Snippet: Inhibition of CDK9 by shCDK9 and LDC000067 represses the cell proliferation and induces apoptosis and cell cycle arrest in HCC cell lines. Bell7402 and HepG2 cells were transfected with sh‐CDK9 and screened with G418 for 2–4 weeks to establish the stable cell lines or treated with 15 μmol/L LDC000067 for 48 h, then the cell proliferation (A and B) was examined by CCK‐8 assays. The apoptosis (C–J) and the cell cycle phase distribution (K–L) were detected by flow cytometry. * P < 0.05.

    Article Snippet: LDC000067 (CDK9 inhibitor) was purchased from Amquar Company (Shanghai, China). sh‐CDK9 plasmid was synthesized by GeneChem Company (Shanghai, China).

    Techniques: Inhibition, Transfection, Stable Transfection, CCK-8 Assay, Flow Cytometry